LumiRDT
LumiSTAR
Real-time Detection Technology
LumiSTAR
LumiRDT
LumiRDT is a collection of gene-edited fluorescent labels or probes for
- Highlighting sub-cellular structures, or
- Detecting intracellular activities
in living cells, packed as ready-to-use reagents for easy operation.
Characteristics of LumiRDT
| LumiRDT Technology | Conventional Probes | |
|---|---|---|
| Nature | Gene-encoded Protein | Chemical |
| Support Detection Duration Window | Weeks | Hours |
| Toxicity | Low | High |
| Label Targeting | Targeting Peptide | Chemistry |
| Customization | – Wavelength – Targeting – Viral Vector | n/a |
LumiRDT-Indicator
Support Real-Time Cellular Activity Monitoring in the Long Term
Example: LumiRDT Ca2+ Indicator on iPSC-derived Cardiomyocytes
Chemical dyes induce cytotoxicity and impair cardiomyocyte beating
Catalogue
| Cell/Sub-cellular Localization | Excitation (nm) | Emission (nm) | Kd | Cat. No | ||
|---|---|---|---|---|---|---|
| ER-LAREX-GECO | ER/SR | 480 | 590 | 560 uM | AA001C-S orL | Calcium Indicator |
| ER/SR | 480, 590 (ratio) | 600 (ratio) | 560 uM | Calcium Indicator | ||
| Mt-LAR-GECO | Mitochondria | Cy3 filter set (ex561/em 594) | 60 uM | AL002C-S or L AL002E-S or L | Calcium Indicator | |
| K-GECO | Cytosol | Cy3 or Texas Red filter set (ex 568/em 594) | 165 nM | AL003C-S or L | Calcium Indicator | |
| NIR-GECO1 | Cytosol | Cy5 filter set (ex 678/ em 704) | 885 nM | AV004C-S or L | Calcium Indicator | |
| NIR-GECO2G | Cytosol | Cy5 filter set (ex 640/ em 685) | 480 nM | AV005C-S or L | Calcium Indicator | |
| G-GECO | Cytosol | FITC filter set (ex 498/em 512) | 620 nM | AL007C-S or L AL007E-S or L | Calcium Indicator | |
| mNG-GECO | Cytosol | FITC filter set (ex 488/em 510) | 807 nM | AL008C-S or L AL008E-S or L | Calcium Indicator | |
| ROS-ROGFP | Cytosol | DAPI (ex 405) FITC (ex 488) | FITC (em 510) | BL001E-S or L | Redox Indicator | |
| MtROS-ROGFP | Mitochondria | DAPI (ex 405) FITC (ex 488) | FITC (em 510) | BL002E-S or L | Redox Indicator | |
| perROS-ROGFP | Peroxisome | DAPI (ex 405) FITC (ex 488) | FITC (em 510) | BL003E-S or L | Redox Indicator | |
| *Available promoter: CMV(ALXXXC) and EF‐1α (ALXXXE) promoter | ||||||
K-GECO (Ca2+ Indicator)
Cytosolic Measurement
LAR-GECO (Ca2+ Indicator)
Mitochondria
MtROS-ROGFP
Mitocondria
LumiRDT-Labeling
A Rainbow Toolbox is Available for Observation
Catalogue
| Structure | EGFP | mCherry | EBFP |
|---|---|---|---|
| Actin | LL005C-S or L | ||
| Tubulin | LL006C-S or L | ||
| Early Endosomes | LL001C-S or L | ||
| Late Endosomes | LL002C-S or L | ||
| Plasma Membrane | LL003C-S or L | ||
| Golgi | LL011C-S or L | ||
| Endoplasmic Reticulum | LL009E-S or L | LL007E-S or L | |
| Mitochondria | LL010E-S or L | LL008E-S or L | |
| Nucleus | LL004C-S or L | LL012C-S or L | |
| Peroxisomes | LL013C-S or L | ||
| Lysosome | LL014C-S or L | ||
| Cytosol | LL015C-S or L |
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